ii one step cloning kit (Vazyme Biotech Co)
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ii one step cloning kit
Ii One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 6258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ii+one+step+cloning+kit/ClonExpress+II+One+Step+Cloning+Kit/pmc13090716-105-13-18
Average 99 stars, based on 6258 article reviews
Ii One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 6258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ii+one+step+cloning+kit/ClonExpress+II+One+Step+Cloning+Kit/pmc13090716-105-13-18
Average 99 stars, based on 6258 article reviews
ii one step cloning kit - by Bioz Stars,
2026-09
99/100 stars
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Amplification:Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor Article Snippet: .. Additionally, Et MIC2-2 (153-855 bp) and linearized pBiFC-VN173 were amplified from pBiFC- Et MIC2-VN173 using primers containing homologous sequences, followed by recombination using the ClonExpress® Article Title: SLIRP maintains energy metabolism homeostasis in colorectal cancer by stabilizing mitochondrial-encoded mRNAs. Article Snippet: BACKGROUND: Colorectal cancer (CRC) is a highly vascularised tumour often characterised by elevated oxidative phosphorylation (OXPHOS) activity, positioning OXPHOS as a potential metabolic vulnerability for targeted therapy.. SLIRP is an RNA-binding protein involved in the post-transcriptional regulation of mitochondrial gene expression.. However, its specific function and underlying mechanism in CRC remain poorly understood. Cloning:Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor Article Snippet: .. Additionally, Et MIC2-2 (153-855 bp) and linearized pBiFC-VN173 were amplified from pBiFC- Et MIC2-VN173 using primers containing homologous sequences, followed by recombination using the ClonExpress® Article Title: Two-step bioconversion of pyridoxal 5′-phosphate from pyridoxine through cofactor regeneration and pyridoxine 5′-phosphate oxidase evolution Article Snippet: .. The purified DNA fragments were subsequently inserted into the pYB1s via a ClonExpress Article Title: Telomere-to-telomere genome assemblies and population resequencing of diploid and allotetraploid peanut varieties. Article Snippet: .. The CDSs of AhWRI1 and AhGSA1 without stop codons were cloned into the pCAMBIA1302 vector using a ClonExpress Article Title: SLIRP maintains energy metabolism homeostasis in colorectal cancer by stabilizing mitochondrial-encoded mRNAs. Article Snippet: BACKGROUND: Colorectal cancer (CRC) is a highly vascularised tumour often characterised by elevated oxidative phosphorylation (OXPHOS) activity, positioning OXPHOS as a potential metabolic vulnerability for targeted therapy.. SLIRP is an RNA-binding protein involved in the post-transcriptional regulation of mitochondrial gene expression.. However, its specific function and underlying mechanism in CRC remain poorly understood. Article Title: FOXC2 represses NFAT1-dependent transcription through a DNA-facilitated protein–protein interaction Article Snippet: .. All plasmids were constructed through homologous recombination technology according to the manufacturer’s instructions for ClonExpress Article Title: Genome-Wide Identification and Evolutionary Analysis of Sucrose Synthase ( SUS ) Gene Family in U's Triangle Brassica Species. Article Snippet: Vector Construction and Arabidopsis Transformation The complete coding sequence (CDS) of BnSUS3-2 was amplified using gene-specific primers (Table S1). .. The resulting PCR products were subcloned into the pCAMBIA1300 vector, which had been linearized by digestion with KpnI and BamHI, using the ClonExpress® Article Title: Molecular characterization and functional analysis of thioredoxin peroxidase-2 from Babesia duncani. Article Snippet: Human babesiosis caused by Babesia duncani is an emerging zoonotic disease that poses a growing threat to public health.. Since the first reported case in 1991, knowledge of this pathogen has remained limited.. Recent completion of the B. duncani genome sequence has accelerated research aimed at elucidating its molecular biology. Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach Article Snippet: Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress Purification:Article Title: Two-step bioconversion of pyridoxal 5′-phosphate from pyridoxine through cofactor regeneration and pyridoxine 5′-phosphate oxidase evolution Article Snippet: .. The purified DNA fragments were subsequently inserted into the pYB1s via a ClonExpress Clone Assay:Article Title: Telomere-to-telomere genome assemblies and population resequencing of diploid and allotetraploid peanut varieties. Article Snippet: .. The CDSs of AhWRI1 and AhGSA1 without stop codons were cloned into the pCAMBIA1302 vector using a ClonExpress Article Title: Molecular characterization and functional analysis of thioredoxin peroxidase-2 from Babesia duncani. Article Snippet: Human babesiosis caused by Babesia duncani is an emerging zoonotic disease that poses a growing threat to public health.. Since the first reported case in 1991, knowledge of this pathogen has remained limited.. Recent completion of the B. duncani genome sequence has accelerated research aimed at elucidating its molecular biology. Plasmid Preparation:Article Title: Telomere-to-telomere genome assemblies and population resequencing of diploid and allotetraploid peanut varieties. Article Snippet: .. The CDSs of AhWRI1 and AhGSA1 without stop codons were cloned into the pCAMBIA1302 vector using a ClonExpress Article Title: SLIRP maintains energy metabolism homeostasis in colorectal cancer by stabilizing mitochondrial-encoded mRNAs. Article Snippet: BACKGROUND: Colorectal cancer (CRC) is a highly vascularised tumour often characterised by elevated oxidative phosphorylation (OXPHOS) activity, positioning OXPHOS as a potential metabolic vulnerability for targeted therapy.. SLIRP is an RNA-binding protein involved in the post-transcriptional regulation of mitochondrial gene expression.. However, its specific function and underlying mechanism in CRC remain poorly understood. Article Title: Genome-Wide Identification and Evolutionary Analysis of Sucrose Synthase ( SUS ) Gene Family in U's Triangle Brassica Species. Article Snippet: Vector Construction and Arabidopsis Transformation The complete coding sequence (CDS) of BnSUS3-2 was amplified using gene-specific primers (Table S1). .. The resulting PCR products were subcloned into the pCAMBIA1300 vector, which had been linearized by digestion with KpnI and BamHI, using the ClonExpress® Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach Article Snippet: Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress Construct:Article Title: FOXC2 represses NFAT1-dependent transcription through a DNA-facilitated protein–protein interaction Article Snippet: .. All plasmids were constructed through homologous recombination technology according to the manufacturer’s instructions for ClonExpress Homologous Recombination:Article Title: FOXC2 represses NFAT1-dependent transcription through a DNA-facilitated protein–protein interaction Article Snippet: .. All plasmids were constructed through homologous recombination technology according to the manufacturer’s instructions for ClonExpress Polymerase Chain Reaction:Article Title: Genome-Wide Identification and Evolutionary Analysis of Sucrose Synthase ( SUS ) Gene Family in U's Triangle Brassica Species. Article Snippet: Vector Construction and Arabidopsis Transformation The complete coding sequence (CDS) of BnSUS3-2 was amplified using gene-specific primers (Table S1). .. The resulting PCR products were subcloned into the pCAMBIA1300 vector, which had been linearized by digestion with KpnI and BamHI, using the ClonExpress® Article Title: Molecular characterization and functional analysis of thioredoxin peroxidase-2 from Babesia duncani. Article Snippet: Human babesiosis caused by Babesia duncani is an emerging zoonotic disease that poses a growing threat to public health.. Since the first reported case in 1991, knowledge of this pathogen has remained limited.. Recent completion of the B. duncani genome sequence has accelerated research aimed at elucidating its molecular biology. Ligation:Article Title: Molecular characterization and functional analysis of thioredoxin peroxidase-2 from Babesia duncani. Article Snippet: Human babesiosis caused by Babesia duncani is an emerging zoonotic disease that poses a growing threat to public health.. Since the first reported case in 1991, knowledge of this pathogen has remained limited.. Recent completion of the B. duncani genome sequence has accelerated research aimed at elucidating its molecular biology. Transformation Assay:Article Title: Molecular characterization and functional analysis of thioredoxin peroxidase-2 from Babesia duncani. Article Snippet: Human babesiosis caused by Babesia duncani is an emerging zoonotic disease that poses a growing threat to public health.. Since the first reported case in 1991, knowledge of this pathogen has remained limited.. Recent completion of the B. duncani genome sequence has accelerated research aimed at elucidating its molecular biology. Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach Article Snippet: Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress Sequencing:Article Title: Molecular characterization and functional analysis of thioredoxin peroxidase-2 from Babesia duncani. Article Snippet: Human babesiosis caused by Babesia duncani is an emerging zoonotic disease that poses a growing threat to public health.. Since the first reported case in 1991, knowledge of this pathogen has remained limited.. Recent completion of the B. duncani genome sequence has accelerated research aimed at elucidating its molecular biology. Recombinant:Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach Article Snippet: Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress Transfection:Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach Article Snippet: Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress Positive Control:Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach Article Snippet: Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress |